The nutritional and medicinal benefits of vegetables provide a better support for human wellbeing. There are several edible vegetables which are used in day to day kitchen in different forms. The usages of green leafy vegetables are limited to a specific geographical location. In the present work, we have investigated phytochemicals of Spinacia oleracea, one of the most important vegetable used in India. The presence of phytochemicals including phytosterols, saponins, alkaloids, phenolic compounds and tannins, proteins, glycosides, flavonoids, carbohydrates, quinones, coumerin, terpenoids, anthocyanins and emodins were determined in the Spinacia oleracea. The extract of Spinacia oleracea exhibits the presence of phytosterols, proteins, glycosides, flavonoids, carbohydrates, quinones, and terpenoids. It was concluded that the extracts of Spinacia oleracea consists of important constituents for pharmacological activities.