Phytochemical examination on the petroleum ether extract of lichen Parmelia perlata led to the isolation of tridecyl myristate (I), 3-ketooleanane (II), icosan-1-ol (III) and (+) usnic acid (IV). Compounds I-III are isolated first time from this lichen. The structure of these compounds was elucidated on the basis of different spectroscopic techniques.