Plant-derived substances have recently become of great interest owing to their versatile applications. The species Carduus chevallieri (C. chevallieri) is a traditional medicinal plant used to treat abdominal disorder, wounds, diabetes and hypertension among the peoples of Southern Nations Nationalities Peoples Regional State, Ethiopia. The chemical constituents of C. chevallieri, however, have not yet investigated although it has a wide traditional use for different ailments. The aim of this study was to carry out phytochemical screening, compound isolation, and structure elucidation of compounds isolated from the root of C. chevallieri.A standard phytochemical screening method was used to investigate the presence or absence of secondary metabolites, in the crude extracts. Compound isolation was performed using chromatographic separation techniques. Spectral data for structure elucidation of the isolated compounds were obtained by using IR, 1H-NMR and 13C-NMR spectroscopic techniques. The structure elucidation of the compounds was performed by interpretation and analyzing the IR, 1H-NMR and 13C NMR spectral data and comparison with literature reports. The root of C. chevallieri was collected from Angacha town, Kembata Tembaro Zone, South Nations and Nationalities Regional State, Ethiopia. The crude extracts were obtained by maceration technique by drying the root (500 g) under shed, finely grinding, and soaking the root powder in solvents n-hexane chloroform, acetone, chloroform/methanol and methanol, sequentially. The solvent was removed by rotary evaporator and the extracts were recovered. The phytochemical screening tests of the chloroform and methanolic extracts revealed the presence of cardiac glycosides, terpenoids, steroids, tannins, alkaloids, flavonoids, anthraquinones, saponins and phenols. Syringin and stigmasterol were isolated from the methanolic extract. Other than the two isolated compounds, its richest bio-resource of several bioactive secondary metabolites that can be used as candidates in drug discovery and development programs. This makes the species a valuable medicinal plant.
