The main objective of this work was to isolate and characterize the natural products from stem of Sapium ellipticum. This powdered plant material was gradiently extracted using petroleum ether, chloroform and acetone using a soxhlet extractor. The chloroform extract was subjected to column chromatography over silica gel using petroleum ether and ethyl acetate as eluents. Three compounds were isolated in the pure form. The IR, 1H NMR, 13 C NMR and DEPT-135 spectra of the compounds were recorded. A pair of broad singlet protons appeared in the 1H NMR spectra at δ 4.604 and 4.730(1 H each) for compound 1. Similar peaks at δ4.587 and 4.709(1H each) were seen for compound 2 also. This was indicative of the exomethylene group present in the case of lupane class of triterpenoids. Compound 1 was characterized as Lupeol acetate and compound 2 as Lupeol based upon on the other significant data from 1H NMR, 13C NMR and DEPT-135 spectrum and also from comparison of this obtained data with published values. The 1H NMR spectrum of compound 3 showed characteristic values to that for a plant sterol. The13C-NMRspectrum of compound 3 showed the presence of 29 carbons with four olefinic carbons at δ 121.9(C-6), 141.0 (C-5), δ 138.5(C-22) and 129.5 (C-23). Compound 3 was characterized as stigmasterol based upon the physical and spectral data. All the compounds are isolated from this plant for the first time.
