The alcoholic extract of the stem bark of Dalbergia sissoo was evaluated for analgesic activity using tail flick method on Wistar Rats. The ethanolic extract was prepared by using soxhlet apparatus. Three different doses (300 mg/ kg, 500 mg/kg and 1000mg/kg) in 0.5 % CMC (carboxyl methyl cellulose) were administered by p.o. route, 300 mg/kg, 500 mg/kg failed to alter pain threshold capacity but increased significantly at the dose of 1000 mg/kg at 30 min. But the results were significant (p < 0.01) for analgesic activity at the all doses at 1 hr. Asprin (300mg/kg) by p.o. route used as standard. Phytochemical investigation showed that ethanolic extract contained carbohydrates, proteins, amino acids, phenolic compounds, and flavanoids. The ethanolic extract showed significant dose dependent analgesic activity. These findings support the use of this drug Dalbergia sissoo in the treatment of pain. The analgesic activity of the bark extract of Dalbergia sissoo may be due to the presence of phytochemical constituents such as flavanoids. The acute toxicity study revealed that ethanolic extract was not toxic up to 3000 mg/kg body weight.