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Phytochemical characterization and antimicrobial activity of Senna alata leaf and flower extracts with cytotoxicity against osteosarcoma cell line | Abstract
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Abstract

Phytochemical characterization and antimicrobial activity of Senna alata leaf and flower extracts with cytotoxicity against osteosarcoma cell line

Author(s): Nidhi Singh, V. Vinothini and Jayanthi Abraham

The present work was aimed at screening for bioactive compounds with pharmaceutical properties from Senna alata flowers and leaves extract. The dried samples were powdered and extracted with ethanol (leaves) and methanol (flower) using Soxhlet apparatus. Both antibacterial and antifungal activities of the extracts were checked against different clinical pathogens. Thin layer chromatography (TLC) revealed the number of compounds present the each extract. The extracts were subjected to GC-MS analysis and phytochemical analysis for phenol, flavonoids, carotenoids etc. were performed to evaluate the biochemical nature of the extracts. Furthermore, cytotoxicity assay were done against osteosarcoma (MG-63) cell lines. The results suggest that they possess anticancer property and antimicrobial activity against Staphylococcus aureus, Klebsiella pneumoniae, Serratia marcescens, Salmonella sp., Penicillium sp., and Aspergillus sp. They were also able to moderately inhibit cell growth of MG 63 cell lines carcinoma.