In the present study phytochemical analysis, antibacterial activity and alpha-amylase inhibitory potential of the traditional polyherbal Siddha formulation was investigated. Different extracts of the polyherbal formulation was prepared and tested for preliminary phytochemical analysis and in vitro antibacterial activity against reference strains. In addition, alpha-amylase inhibitory assay of the herbal formulation were performed in aqueous extract. Secondary metabolites such as saponin, alkaloids, tannins, flavonoids, phenolics and terpenoids were found to be present in aqueous extract of herbal formulation. Mild inhibition on growth of microbes was observed in antibacterial assay. Acetone extract showed 14mm zone of inhibition against Klebsiella pneumoniae. The aqueous extract exhibited up to 69.8% of inhibition of alpha amylase enzyme. Presence of various secondary metabolites in extracts was found to be responsible for antibacterial activity against tested microbes. This study also shows that the herbal formulation was moderately inhibiting alpha amylase enzyme and therefore it may be used as hypoglycemic agents in the management of post prandial hyperglycemia.
