To investigate the antimicrobial activity and phytochemical screening Ethanol, methanol, Ethyl acetate, acetone, chloroform, Petroleum ether, hexane, hot water, and extracts of Withania somnifera. The aim of the present study was to evaluate the qualitative analysis of phytochemicals and antimicrobial activity of various solvent extracts of Withania somnifera. The antimicrobial activities of different solvent extracts of Withania somnifera were tested against the bacterial strains and fungus by observing the zone of inhibition. The Gram-positive bacteria used in the test were Staphylococcus aureus, Bacillus cereus and Micrococcus luteus, and the Gram-negative bacteria were Escherichia coli, Pseudomonas aeruginosa and Klebsiellapneumoniae, fungus like Aspergillusniger, Aspergillusflavus, Candida albicans, Candida tropicalis, Cryptococcus neoformans, and Candida kefyr. It was observed that ethanol; methanol, ethyl acetate, aceton, chloroform, petroleum ether, hexane and aquas extract showed activity against bacteria and fungus. The Ethanol extract of Withania somnifera showed more activity against Staphylococcus aureus, zone of diameter 20.10±0.17 mm and Methanol extract of Withania somnifera showed more activity against Candida albicans, zone of diameter 14.20±0.40 mm compared to other solvent extracts. In this study Ethanol extract in bacteria and methanol extract in fungus showed a varying degree of inhibition to the growth of tested organism, than Ethyl acetate, chloroform, aceton, Petroleum ether, hexane and hot water extracts.
