Sudan is one of the large agriculture- based countries. In this study activated carbon was prepared using two agriculture waste of Sudanese seeds namely Corn maize and Doum fruit . The carbonization of the materials was done at 350oC for two hours and allowed to cool at room temperature then followed by activation withKOH . The results showed that the carbon percentage of doum fruit and Corn maize were 35.13% 49.42 respectively. Porosity of both samples were is in the range from 57-60%, Fourier- Transform Infrared Spectroscopy was used to determine the functional groups of both samples. The results showed a strong and broad adsorption peak at1577.66cm-1, which corresponds to the lactone in doum samples, Conjugate carbonyl was observed at 1595.20 cm-1 for the same sample. The main functional groups of C-O and aliphatic CH2 were found in corn maize sample. Physical properties of activated carbon prepared from doum fruit indicate a pH of 7.8 and volatile matter of 24.3% while activated carbon prepare from corn maize has a pH of 7.6 and volatile matter of 23.0%. Corn maize activated carbon showed a better decoloring activity as it was able to remove 54 % of methylene blue color within 24 hours. On the other hand activated carbon from doum samples showed a better antibacterial activity against E. Coli in contaminated water samples.