Aim of present investigation was to evaluate wound healing activity of Honey, Daruhaldi and Shatdhautghrut singly and in combination on experimentalexcision wound healing activity in rats. Incisions wounds were used to study the topical effect of Honey, Daruhaldi and Shatdhautghrut singly and in combination for 20 days (once a day) on various biochemical parameters. Honey + Daruhaldicombination treatment showed significant increase (p <0.01) in tensile strength, hydroxyproline content and total protein level as compared to vehicle control rats. Daruhaldi showed more significant (p <0.01) comparable effect in increasingtensile strength, hydroxyproline content and total protein level as compared to vehicle control rats as well as Honey + Shatdhaut or Honey orShatdhaut. When compared with vehicle control rats, topical administration of Honey + Shatdhaut or Honey orShatdhaut showed significant increase (p <0.05) in tensile strength, hydroxyproline content and total protein level. In conclusion, oral administration of honey, Daruhaldi and Shata-dhauta-ghrita as well as their combinations promotes faster wound healing by acting simultaneously favorably on various processes of wound healing such as collagen synthesis and epithelization. The presence of flavonoid moities in honey and Daruhaldi combination showed better wound healing potential than Honey &Daruhaldi alone.
