The Study was mainly aimed to establish the local ethno-medicinal claim of Assam that lemon peel oil has Antimicrobial property. Again when searching for the Microscopic Characters of lemon leaf for identification, data’s were not available easily and then the aim of the study was broadened to include evaluation of pharmacognostic, phytochemical, physicochemical property and to make the data’s available easily for further use. The Lemon Peel Oil as every other volatile oil has shown antimicrobial activity, but was active against both Gm +ve and Gm _ve organism. When compared with Standard drug Amoxicillin the activities were less potent but can be useful if explored either alone or in combination with other antimicrobial agents.