Numerous studies show that there is relationship between skilled performance and body organs dominants, such as hands, feet and eyes. In order to compare the performance of left and right basketball lay-up in ipsilateral and contralateral conditions of hands and feet in male students of Tabriz Islamic Azad University, 100 male student were selected by a described questionnaires, Chapman - Chapman hand superiority and Wai - Hong Jackie Lam foot superiority and were randomly divided into four groups: 1) 30 right ipsilateral, 2) 30 left ipsilateral, 3) 20 right contralateral and 4) 20 left contralateral. Participants tried left and right lay-up in 150 trials for 5 days and finally 10 efforts for an optimal test were conducted. Data was analyzed by variance of 2 × 4 combined design within and between groups, and Bonferroni test. By analyzing the proposed hypotheses at the P≤ 0.01 demonstrated significant differences among groups in performance. In general, contralateral students, especially right contralaterals had better performance compared with other groups