The aim of this experimental study was to isolate and partially purify extracellular xylanase from Penicillium janthinellum and Neurospora crassa.The species were inoculated in a xylanase fermentation medium .The supernatants were collected after 5 days. The partial purification was realized by applying respectively, ammonium sulfate precipitation, dialysis and DEAE-Cellulose ion exchange chromatography to the supernatant. Effect of pH and temperature on enzyme activity and stability were determined. In addition, the molecular mass of the obtained enzyme was investigated by Sodium Dodecyl Sulphate-Polyacrylamide gel electrophoresis (SDSPAGE). The final enzyme preparation from Penicillium janthinellum and Neurospora crassa were 6.5 and 2.2 fold more pure than the crude homogenate respectively. The molecular mass of the partially purified enzyme from Penicillium janthinellum and Neurospora crassa were found to be 39kDa by using SDS-PAGE.
