Microorganisms are a source of many biotechnological products in modern world. Antibiotics are one of the secondary metabolites produced by some fungi and bacteria. The aim of this study was to determine the optimal conditions for penicillin production and extraction from the Penicillium chrysogenum culture filtrates. Two isolates of the fungus were subjected to experiments. Metabolites were detected using TLC and HPLC compared with the penicillin G standard . It was found that, maximum production of penicillin in broth culture media was the interval of 8-6 days after inoculation. Optimal culture conditions was the presence of 3 g yeast extract, 21 g of sucrose per liter ,the optimal temperature conditions between 28-25 ° C with 120 rpm of the Shaker movement. No meaningful differences were observed between two tested isolates.
