Citric acid ,an industrial organic acid produced by Aspergillus niger from cassava peel with different carbon and nitrogen sources. Glucose enriched medium showed high yield of citric acid. Ammonium chloride and ammonium persulphate reduced the production of citric acid. The maximum yield of citric acid was observed in ammonium dihydrogen phosphate. The optimum yield of citric acid from cassava peel using aspergillus niger was found in 20 days of fermentation with pH3 and room temperature.