Single crystals of Thiourea added L-Histidine (TLH) have been grown by slow evaporation solution growth technique from its aqueous solution. To identify the morphology and structure, the as grown crystals were subjected to single crystal x-ray diffraction analysis. The different modes of vibration present in the crystal was identified with FT-IR. The optical transmission spectrum and Second Harmonic Generation (SHG) have been studied to find its linear and Non-linear properties. The thermal stability of the grown crystal was found by differential scanning calorimetry (DSC). To check the mechanical strength Vicker’s microhardness test was carried out and various parameters such as fracture toughness (Kc), elastic stiffness constant (C11) and yield strength (σv) were calculated.