This research is focuses on the effect of doping with Iodine (I2) and Sodium-borohydride (NaBH4) on optical properties of melanin pigment. Thin films of pure melanin and melanin doped with ((I2) and (NaBH4)) have been prepared by spray on evaporator method. The optical properties were characterized by 300 – 800 nm wavelength range measurement. The optical characteristics indicate that the melanin doped with (NaBH4) exhibited the lower absorbance, broaden band gap and high n values, where as the melanin doped with (I2) exhibited the higher absorbance, narrowed band gap and high k values. The energy band-gap of pure melanin was found to be 2.6 eV.