Hospital’sEthical climate is defined as mental structure which is understood by common, fixed and significant understandings. Hospital's ethical climate could affect intra organizational relationships and employees attitudes. Positive ethical climate could reduce the medical errors in hospitals. This study aimed to investigate the Operating room staff attitude toward the ethical climate of educational hospitals of Zahedan University of Medical Sciences. This was a cross-sectional study. We collected the data of this study from all the operating room staff of educational hospitals through Olson's ethical climate questionnaire. Data were analyzed by SPSS v.19 through ANOVA and Ttest. This study was conducted on 71 operating room staff. Age average was 28.34±5 among subjects. There wasn’t any significant relationship between ethical climate and, sex, age, and educational degree. But there was a significant relationship between attitude toward ethical climate and job experience (p=0.05). 57 had bachelor degree and 14 had associated degree. The mean score of ethical climate among staff was 93.11. Present study increases our knowledge about ethical climate of educational hospitals of Zahedan University of Medical Sciences. According to the results of this study operating room staff had a good attitude toward the ethical climate of operating room.