Microscopic examination was used in the research to investigate the prevalence of malaria as well as clinical and parasitological response of Plasmodium falciparum to Artemether-Lumefantrine (AL), Dihydroartemisinin- Piperaquine (DP), Artemether-Injection (AI) and Artesunate monotherapy in 123 people from the urban, semi-urban and rural areas of Kano State, Nigeria. The patients diagnosed with malaria microscopically were treated with one of the aforementioned antimalarials. The parasite shows very low sensitivity to all the drugs used in the study as the treatment failure rate is 70.8% three days of ACT treatment. The results show very high prevalence of suspected artemisinin resistance (88.9% in urban, 45.5% in semi-urban and 100% in rural). Failure of the patients to return to some health facilities after 28 and 42 days of treatment hindered the establishment of Confirmed resistance in some areas. Statistical analysis shows that there is no significant difference in prevalence of malaria; therapeutic efficacy among the artemisinins used in the research as well as the prevalence artemisinin resistance between all the studied areas at p>0.05.
