Pathogenic Vibrios has emerged as a serious and global threat to human health. The research investigates occurrences and diversity of Vibrios species on lettuce and wastewater from Jakara canal that is used to irrigate it.Samples culturedby incubation in alkaline peptone water (APW) and cultured on thiosulfate-citrate-bile-sucrose (TCBS; Merck) agar. Pigmented colonies were Grams’ stained, tested for oxidase, catalase, motility, and indole, cholera red, salt tolerance in (0%, 3%, 6%, 8% and 10% (NaCl) and glucose, sucrose, lactose and arabinose fermentation tests and antibiotics susceptibility profiles were determined. V. cholerae was detected in 11.27% of samples, while V. paraheamolyticus was detected in 12.68%, V. damsel was much less, had 1.41% all of lettuce samples. Similarly, V. fluvialis and V. mimicus were detected each in 2.82% of wastewater. Vibrios species were detected much higher on lettuce than wastewater but no statistical significance (P > 0.05) was observed.Fifty percent (50.00%) of V. cholrae detected from lettuce were resistant to pefloxacin and while V. fluvialis, V. hollisae and V. mimicus were more resistant to nalidixic acid 66.66%, 43.86% and 28.57% respectively. Resistant by V. vulnificus, V. fluvialis, V mimicus and V. paraheamolyticus to ampicilin (33.33%, 33.33%, 25.00% and 16.66% respectively) was also observed. The antibiotic susceptibility patterns were quite similar, and showed statistical significance, at both (P < 0.05) and (P < 0.01) probability levels. Multi-drug resistance was equally observed among all isolates. Theresult demonstrates diverse Vibrio species present in lettuce and wastewater was resistant to antibiotics that should be sensitive to, traditionally. The diversity and resistant patterns were quite a challenge to clinical and public health in future control of Vibrio specie targeted approach.
