cytochrome c oxidase subunit I (cox1) of 655 bp length is used as a barcode region of Osteobrama, Cyprinids (Teleostei:Cypriniformes:Cyprinidae). Cox1 nucleotide sequences for analysis of species identification comes from the degeneracy nature of the genetic code and also; the nature of the highly variable of the amino acids at the third codon position. The most variable region is dominated by amino acids with four or six codons, while the least variable region is dominated by amino acids with two codons. Therefore, cox1 amino acids sequences diversity is less than the nucleotide sequences diversity. Nucleotide sequence of Osteobrama species of 655 bp for cox1, were sequenced. All the species showed different cox1 nucleotide sequences and hence successfully barcode the region. The average genetic distance within the species ranges from 0% to 22% with the overall mean diversity of 18%
