Der Pharmacia Lettre
Abstract
Author(s): Nilesh Jain, Ruchi Jain, Navneet Thakur, Brahm Prakash Gupta, Jitendra Banweer and
Surendra Jain
A novel, safe, accurate and sensitive spectrophotometric method was developed using containing
mixture of 2 M sodium acetate and 8 M Urea in the ration of 50:50% V/V solution as hydrotropic
solubilizing agent for the quantitative determination of poorly water-soluble mixed hydrotropic
solution torsemide , a very slightly water soluble (5.96X10-02 mg/ml) diuretic drug in tablet dosage
form. There were more than 86 fold enhancements in the solubility of torsemide increases in
mixed hydrotropic solution as compared to solubility in distilled water precluding the use of
organic solvents. Torsemide shows maximum absorbance at 288 nm. Sodium acetate, urea and
other commonly used tablet excipients did not show any absorbance above 240 nm, and thus no
interference in the estimation was seen. Torsemide is obeyed Beer�¢����s law in the concentration
range of 10 to 50�Ž�¼g/ml (r2= 0.9996) in mixed hydrotropic solution with mean recovery ranging
from 99.23 to 99.64%. The present investigation is new, simple, economic, safe, rapid, accurate
and reproducible. The developed methods were validated according to ICH guidelines and result
of accuracy, precision and other statistical analysis were found to be in good accordance with
the prescribed values.
