Biosynthesis of gold nanoparticles using Aloe barbedensis leaf extract was studied considering different parameter like pH (3, 4, 6, 8, 10), Temperature (4, 30, 60 and 1000C) and concentration of aurochlorate solution. At lower pH and temperatures, formation of hexagonal and triangles of gold nanoparticles occurred, as viewed by TEM. The leaf extract was so efficient that change in color of reactant was noticed in < 2 minutes of interaction with aurochlorate solution at both low and high temperature. Formation of nanoparticles was characterized by UV-Vis Spectroscopy, XRD and TEM; which confirmed the synthesis of monodispersed gold nanoparticles. Gold nanoparticles synthesised using A.barbadensis was extremely stable as compared to chemically synthesized Gold nanoparticles synthesized using using citrate reduction (chemical) method.