New UV spectrophotometric (UV method) for the quantitative estimation of rebamipide, a potent antiulcer agent and tramadol, a centrally acting analgesic in pure form and in solid dosage forms was developed in the present study. The linear regression equations for rebamipide was Abs=0.09944Conc. (in μg/mL)+0.022 and tramadol was Abs=0.0059Conc. (in μg/mL)+0.003 measured at 228 nm and 271 nm respectively. The detection limit for rebamipide and tramadol was found to be 0.27μg/mL and 0.24μg/mL. The results of analysis were treated statistically, as per ICH guidelines for validation of analytical procedures, and by recovery studies.
