A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of Repaglinide in Bulk and Pharmaceutical tablet Formulation. Isocratic elution at a flow rate of 0.3ml/min was employed on symmetry Shimadzu LC-20 ATVP Kromasil C-18 column Column at ambient temperature. The mobile phase consisted of Methanol : Orthophosphoric acid(1%) : Acetonitrile (30:30:40v/v). The UV detection wavelength was 245nm and 20 μl sample was injected. The run time for Repaglinide is 6 min. The flow rate was found to be 1ml/min. The Average percentage recovery of the method was in the range of 96.1 – 102.6%.The method was validated as per the ICH guidelines. The method was successfully applied for routine quality control analysis of pharmaceutical formulation. The HPLC method can be successfully applied for the routine quality control analysis of Repaglinide formulations, which could be the better choices compared to the reported methods of literature.
