Sea buckthorn is a neglected multipurpose plant in Pakistan with around 300 hectares of its wild populations. The leaves and berries of two sea buckthorn genotypes (H. turkestanica and H.sinensis) were evaluated for mineral composition, phenolic contents and antioxidant and anti-coagulant activities for the 1st time from Pakistan. Antioxidant activities were estimated by measuring the scavenging activities of leaf extracts against 2,2-Diphenyl-1- Picrylhydrazyl (DPPH) radicals. Amongst the two genotypes of leaves, H. turkestanica showed 87% anti oxidant activity as compared to 95% in berries of H. turkestanica. The higher range of biochemical constituents. i.e. vitamin C 83.6 mg100 g-1, phosphorous 41.5 mg100 g-1, iron 78.1 mg100 g-1, magnesium 56.8 mg100 g-1 and calcium 18.6 mg100 g-1 in leaves was observed in H. turkestanica. The total phenolic contents were 19.03 mg GAE/g in leaves of H. turkestanica thus comparatively higher than H. sinences. i.e.16.3 mg GAE/g. The in-vitro anticoagulant activity of Sea buckhorn berries acetonic extract in inhuman blood was showed very promising results and clotting time was noted upto 45 minutes which is higher than normal blood clotting time. The study revealed that Sea buckthorn leaves and berries have significant potential for utilization in food and pharmaceutical industry in Pakistan sustain the rural economy of mountain areas.
