In the present study the mudskipper (Gobidae: oxudercinae) fauna of estuarine region of Northern Gulf of Kahmbhat, Gujarat, India is studied. The specimens were collected from three different sites viz Kamboi, Nada and Gandhar located on the northern part of Gulf of Khambhat during low tide using hand picking method. Total four different species of mudskippers were identified that includes Boleophthalmus dussumieri Valenciénnes, 1837, Periophthalmus waltoni Koumans, 1955, Apocryptes bato (Hamilton, 1822) and Scartelaos histophorus (Valenciennes, 1837). Amongst the four species reported B. dussumieri and P. waltoni are commonly observed in the estuarine and mangrove mudflats of Gujarat state while A. Bato and S. histophorus are reported first time from Gujarat state. The detailed description of all the species is given in the report.