The objective of the present investigation was to formulate and evaluate the mucoadhesive microsphere of Glipizide using Hydroxyl Propyl Methyl Cellulose K4M and Carboxy Methyl Cellulose as polymers. Glipizide microspheres were prepared by simple emulsification phase separation technique using glutaraldehyde as a cross linking agent. Twenty preliminary trial batches, F1-F20 batches of microspheres were prepared by using different volume 10 to 70 ml of glutaraldehyde as cross linking agent, cross linking time 1 to 4 hours and 3:1 ratio of polymerto- drug with two different polymers. From these twenty batches of each polymer, the optimized formulation is selected based on the percentage of mucoadhesion, Drug entrapment efficiency and sphericity of microspheres. A 32 full factorial design was employed to study the effect of independent variables, polymer-to-drug ratio (X1), and stirring speed (X2) on dependent variables percentage of mucoadhesion, drug entrapment efficiency, swelling index and invitro drug release study. The drug polymer compatibility studies were carried out using FTIR and the stability studies were conducted for the optimized formulation. Among the two polymers, the best batch was Hydroxy propyl methyl cellulose K4M exhibited a high drug entrapment efficiency of 69% and a swelling index 1.16 % mucoadhesive after 1hour is 70% and the drug release was also sustained for more than 12 hours. The polymer-to-drug ratio had a more significant effect on the dependent variables.
