Methicillin-resistant Staphylococcus aureus one of the main pathogen responsible for causing infections of skin and systemic infections. This methicillin-resistant Staphylococcus aureus may be resistant to β-lactam antibiotics as well as other antibiotics. The resistance of Methicillin-resistant Staprhylococci to various was revealed by antibiotic sensitivity test and the gene for antibiotic resistance was revealed by carrying out PCR using mecA and blaIMP primers. The molecular characterization and antibiotic sensitivity test was carried out for the three strains collected from the ocular patients. The MIC test was carried out for all the three strains of Staphylococci using vancomycin which revealed the minimum concentration of vancomycin required to inhibit the growth of this pathogen.