Boerhaavia diffusa is one of the well-known traditional medicinal plants. They are rich source of secondary metabolites like alkaloids, steroids and flavones. The chief function of flavonoids in plant includes UV filtration, symbiotic nitrogen fixation, and floral pigmentation. They also act as chemical messengers, cell cycle inhibitors and physiological regulators. The present study was focused on the detection and quantitative estimation of flavonoids in the in vitro plant cell suspension culture. The quantitative estimation showed that there was an increase in flavonoid production with increase in UV irradiation time, heavy metal concentration and number of days.
