Rutin is the natural flavonoid with antihyperglycemic, antihypertensive, and antioxidative properties. To study in vitro production of rutin, we established plant tissue culture techniques for micropropogation of Alpinia purpurata. The specific objectives of this study were to test some of the simple methods and cheaper alternatives for micropropagation of Alpinia purpurata for determination of rutin. The accumulation of flavonoids was evaluated in organogenic cultures of Alpinia purpurata using High Performance Thin layer Chromatography (HPTLC). Alpinia purpurata were cultured on Murashige and Skoog, 1962 (MS) agar medium supplemented with low cost alternatives like coconut water, marketed sugar, commercial corn flour as alternative gelling agents, benzylaminopurine (BA), Kinetin, NAA (Napthalene-3-acetic acid), IAA (Indole-3-acetic acid) and 2, 4-D (2, 4-Dichlorophenoxyacetic acid) at the different concentration range for callus, shoots and root initiation. With a combination of low cost additives in MS media the best result of Alpinia purpurata was found in 2, 4-D (2 ppm) and kinetin (2ppm) for callus initiation, maximum number (9-11) of shoots were observed in medium with NAA (0.1ppm) and BA (3.0 ppm). Roots initiation was found in IAA at the concentration of 3ppm. The results of HPTLC methods revealed that rutin content in the leaves extract of Alpinia purpurata was more in low cost tissue culture grown plant micropropogated with 20% coconut water, 3% marketed sugar and 100 gm/l gelling agent than naturally grown plants. It indicate that low cost media in tissue cultures of Alpinia purpurata could be a valuable alternative approach for rutin production
