Microbiological quality of remnants of Sodium bicarbonate, potassium chloride and gentamicin single dose ampoules used in emergency paediatric unit of University of Maiduguri Teaching Hospital was investigated. Nutrient agar, MacConkey agar, Saboraud dextrose agar, Mannitol salt agar, tryptone soya broth and thioglycollate medium were used for isolation and differentiation of microorganisms. Three methods (uncovered, covered with cotton wool and covered with plaster) of storage of the remnants were evaluated. After 8 hours of storage, contamination began to manifest starting with the uncovered, cotton wool covered then plaster covered. The vulnerability to microbial contamination was found to be Sodium bicarbonate> potassium chloride> gentamicin. The contaminating organisms include yeast and Staphylococcus aureus. Single dose ampoules should be used only once
