A Simple, accurate and precise method for estimation of reversed phase HPLC method has been developed for the simultaneous determination of sitagliptin and Metformin by using Hypersil BDS C18 (100 x 4.6 mm, 5μm particle size) column and mobile phase of at 215 nm. A mobile phase has a composition of potassium dihydrogen orthophosphate and methanol(50:50v/v),adjusted the pH 8.5 with o-phosphoric acid was used and flow rate 1.0ml/min. Retention times of Sitaglipitin and Metformin were 2.3 min and 4.6 min respectively. The method developed validated as per ICH guideline
