The objective of this work is to develop a rapid, precise, accurate and sensitive reverse phase liquid chromatographic method for the estimation of nebivolol in the animal studies used valsartan as an internal standard. The chromatographic method was standardized for nebivolol using Shimadzu HPLC model reverse phase analytical Inspire C18 column (250 mm x 4.5 mm, 5 μm particle size) with LC10AD Pump and SPD-10A UVDetector, at 282nm and flow rate of 1ml/min. The mobile phase consists of 50:10:40 acetonitrile: Methanol: Water (0.1%gaa). The linearity of proposed method was investigated in the range of 1-50 μg/ml (R²=0.999) for nebivolol. The limit of detection (LOD) was found to be 0.17μg/ml. The limit of quantification (LOQ) was found to be 1.02μg/ml. The retention time of nebivolol found to be 4.2 min. The method was statistically validated and %RSD was found to be less than 2 indicating high degree of accuracy and precision. Hence proposed method can be successfully applied for the estimation of nebivolol in animal model studies
