A simple, specific, accurate and precise reverse phase high pressure liquid chromatographic method has been developed for the determination of Amlodipine Besylate and Hydrochlorothiazide in bulk and combined dosage form by RP- HPLC using Hypersil BDS C18 column (Dimention : 250mmx 4.6mm, 5μ). The sample was analysed using Acetonitrile:Water 50:50% v/v mobile phase at a flow rate of 1.0ml/min and detection at 254nm. The retention time for Amlodipine Besylate and Hydrochlorothiazide was found to be 1.9 and 3.2 min respectively, and recoveries from combined dosage form were between 100.2% and 99.17%. The method can be used for routine analysis of Amlodipine Besylate and Hydrochlorothiazide in bulk and combined dosage form.
