The study evaluated metal chelating activity of Glycine max seed extract on Doxorubicin (DOX) induced cardiotoxicity in rats. In this study twenty four male Albino Wistar rats weighing between 200-250g were used. The animals were treated as follows. Group I: Animals served as vehicle control and received 0.5% tween 80 (10 ml/kg, p.o). Group II: Animal received Ammonium Ferric Citrate (0.6 mg/kg/day, p.o for 14 days) followed by doxorubicin (10mg/kg, i.v; once 48 hr before sacrifice), Group III: Animal received Deferoxamine 20 mg/kg, p.o. for 14 days followed by ferrous + doxorubicin, Group IV: Animal received Glycine max alcoholic extract 200 mg/kg, p.o for 14 days followed by ferrous + doxorubicin. In each group, body wt of rats were taken before and after doxorubicin administration. After 48 hrs of doxorubicin administration blood was collected for serum CK-MB and LDH estimation. Isolated hearts were dried and weighed. In heart tissues superoxide dismutase (SOD) & catalase (CAT), glutathione (GSH) and malondialdehyde (MDA) were estimated. Results showed that mean heart weight/body weight (HW/BW) ratio in group 2 was significantly (p<0.001) decreased, CKMB (p<0.01), LDH (p<0.05), MDA (p<0.01) increased, GSH (p<0.01), SOD and CAT (p<0.01) were decreased as compared to group 1. Group 3 & 4 has shown significant (p<0.01) increase in (HW/BW) ratio, decrease in CKMB (p<0.01), LDH (p<0.05), MDA (p<0.01), GSH (p<0.05), SOD (p<0.01) and CAT (p<0.01) increased as compared to group 2. It may be concluded that Glycine max alcoholic extract posseses cardioprotective and metal chelating activity in Doxorubicin induced cardiotoxicity in rats.
