Excessive and prolonged exposure to petrol fumes has been shown to cause a significant health hazard which may be multisystemic. Data on liver involvement is highly inconclusive and scanty in this environment hence this study. The present study investigated Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Alkaline phosphatase (ALP), Total bilirubin (T-BIL), Conjugated bilirubin (C-BIL) and Albumin (ALB) as liver function indicators. A total number of ninety (90) subjects, comprising of sixty (60) gasoline attendants (35 males and 25 females) and thirty (30) apparently healthy non gasoline attendants as control (18males and 12 females) were recruited for the study. Analysis of the analytes was carried out using standard spectrophotometric methods. The mean values of ALT, AST, ALP, T-BIL, C-BIL and ALB obtained from gasoline attendants were not significantly different when compared with the controls (p>0.05) although slightly higher. Conclusively, liver function indicators were within normal range in Nigerian gasoline attendants studied.