Quality of life is one of the most important components of health that is affected by different factors. Since the quality of working life is one of the most effective ways to motivate and help designing and enriching employees job; therefore the present study has been done in order to assess the quality of life among the operating rooms personnel working in educational hospitals of Zahedan Medical Sciences University. This is a descriptive - analytical study conducted on 112 subjects who were selected through census. To collect data a questionnaire consisting of two parts was used. The first part was associated with demographic characteristics and the second part was related to the life quality standard questionnaire (SF-36). Finally, after collecting the questionnaires, the data was analyzed using SPSS 19 software and descriptive statistics, Pearson correlation, variance analysis and independent t-test. The results showed that subjects' mean age was 30.95 ± 7.76. Also, 41 subjects were single and 88 subjects were women. In addition, 60 subjects had a 1-5 year work experience, 22 employees had a 5-10 year work experience, 18 subjects had a 10-15 year experience and 12 employees had more than 15 years working experience. Totally, the average score of life quality among employees was 36.58 ± 17.28 indicating the personnel weaknesses at their own life quality. The relationship between marital status, age and work experience with quality of life was not statistically significant (P> 0.05). Due to the fact that nurses' life quality may affect their performance quality and the way they take care for patients; thus, medical- health centers officials must identify factors that result in reducing nurses' life quality and through the implementation of programs such as holding training courses improve their quality of life.