Levels of pollutants in urban stormwater runoff are closely related to various types of land use because human activity is different according to land use. This study assesses the impacts of land use on stormwater quality. Stormwater samples were collected from five different land use types; residential, commercial, recreational, heavy traffic and industrial, around Guwahati city, Assam (India). Each of the collected samples was analysed to measure important water quality parameters such as suspended solids (SS), total residual chlorine, oil and grease, nitratenitrogen (NO3-N) and phosphate (PO4 3-). The analysis shows that the spatial variation in the distribution of pollutants in stormwater was highly influenced by the land use type. In industrial and commercial land use, the pollutants exceeded the guideline values as per Indian National Standards. Overall, the industrial land use site produces the worst quality stormwater. The study also provides recommendations for improvement and management of storm water quality in the city.