Soybean, glycine max, axes is known to contain L-arginase. The present work is based on development of plant arginase based biosensor for the monitoring of arginine in juice samples. The presence of L-arginine in juice samples has been studied by using immobilization techniques such as gelatin, polyacrylamide gel, agar and calcium alginate beads. The response times and stability of L-arginase with different method of immobilization has been studied. The indicator (Phenol red) was coimmobilized with plant arginase and urease. The detection range of 10-10 – 10-1 M was studied. These techniques have been utilized for the detection of arginine in juice samples (10-2- 10-5 M) and maximum concentration was found in orange juice (10-2 M).
