Probiotics are microorganisms that, in sufficient quantity, exert a positive effect on health. They have an important role in improving digestion and bowel function, maintaining the balance of intestinal flora and the acid-base balance in the colon. Probiotic strains must also have good technological properties. Indeed, this study aims to evaluate the probiotic ability of a collection of lactic acid bacteria strains with proteolytic character, isolated from cow's milk and goat local Algerian populations, in different growth conditions (pH survival 2.5 and 2, the resistance to 0.3% bile salts, and evaluating the hydrophobicity of the cell surface cultures against Xylene). All the strains studied and their associations have shown significant resistance against the acid pH and high concentration of bile salts (0.3%). In addition, the percentages of xylene strains adhesion indicates the hydrophobicity of their surfaces. In the end, three strains were selected because they show a most interesting probiotic profile characterized by a strong antibacterial effect against pathogenic strains. They were identified at the molecular level by 16S rDNA sequencing as Lactobacillus plantarum C7, C8 and Lactobacillus casei C5.
