Mucuna pruriens Linn. is a magic medicinal plant used for treatment of Parkinson's disease and many others in ancient Indian medical system. L-DOPA extracted from callus of Mucuna is a constituent of more than 200 indigenous drug formulations and is more effective as drug than the synthetic counterpart. HPTLC analysis of L-DOPA in crude extract of Mucuna pruriens callus confirmed by comparing the standard L-DOPA. The quantification of L-Dopa from callus extract after spiking was found to be in 82.35 % while standard was 88.50%. HPLC analysis of L-DOPA in crude extract of Mucuna pruriens callus confirmed by comparing the standard L-DOPA. The quantification of L-Dopa from callus extract was found to be in 89.48 % while standard was 93.45%.
