The shade dried fruit coat husk powder of Juglans nigra (Wall nut) was subjected to column chromatographic separation which yielded 7 fractions. The fractions were then subjected for different spectral (IR, 1H NMR and MS) and chemical analysis. Nine compounds including two 1,4-napthoquinones derivatives, juglone (I) and plumbagin (II), one α-tetralone derivative, (-)- regiolone (III), two sterols, stigmasterol (IV) and β-sitosterol (V), four flavonoids, taxifolin (VI), kaempferol (VII), quercetin (VIII) and myricetin (IX) were isolated from chloroform fraction of aqueous extract of Juglan nigra husk powder. These extracted compounds were subsequently used for dyeing of Pashmina Shawl as natural dye substances which gave promising results. It was inferred that the combined effect of Jugalone and Plumbagin was responsible for the dyeing behaviour of wall nut husk powder.
