The objective of present work is to study the in-vitro anti-oxidant activities of Joha Rice; an aromatic indigenous rice of Assam, India. Anti-oxidant activity of the extracts were studied using 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, Hyderogen peroxide(H2O2) scavenging activity and Nitric Oxide(NO) scavenging activity. The total Phenolic contents and Flavanoid contents were estimated taking Gallic Acid and Quercetin calibration curve respectably. In in-vitro anti-oxidant studies it was found that ethanolic extract of Joha Rice posses good free radical scavenging activity and was comparable with standard Ascorbic acid. The IC 50 for Joha Rice was found in DPPH scavenging activity 81.45±2.29 μg/ml, Hydrogen Peroxide scavenging activity 121.30±2.20 μg/ml and Nitric Oxide scavenging activity 137.67±3.34. The total Phenolic content was found 74.86±2.474 mg/gm Eq of Gallic Acid and Total Flavanoid content was 190.7±15.28 mg/gm Eq of Quercetin. Ethanolic extract of Joha Rice contains phenolic and Flavanoids and posses anti-oxidant activities in In-vitro methods. So, we can further use this for screening of biological activities in metabolic and degenerative diseases which were cause due to free radical mechanism.
