Chemical investigation of the dichloromethane extract of freeze-dried Raphanus sativus roots led to the isolation of 4-methylthio-3-butenyl isothiocyanate or raphasatin (1a), 4-(methylthio)butyl isothiocyanate or erucin (1b), β- sitosterol (2) and unsaturated triglycerides (3). The structures of 1a and 1b were elucidated by extensive 1D and 2D NMR spectroscopy, while those of 2 and 3 were identified by comparison of their NMR data with those reported in the literature.
