The whole plant of Borreria stachydea[(DC) Hutch. & Dalziel] was collected fresh from Birnin Magaji village, in Zamfara state, Nigeria in November, 2010. Taxonomical identification was done by Mallam Musa Abdullahi at Herbarium unit of Biological Science Department, ABU, Zaria, Nigeria. The whole plant wasair dried under shade, segregated and pulverized. 791.94g of the pulverized plant materials were carefully weighed and loaded into a Soxhlet extractor. It was extracted with Chloroform. 10.0g of the chloroform crude extract was loaded in a column and was developed by gradient elution. The purified sample from the above column chromatography was subjected to preparative TLC. The result gave rise to WIPO 2. WIPO 2 was subjected to spectroscopic analysis for structural elucidation and characterization. The results are shown in Table 1. Gas Chromatography – Mass Spectroscopy (GC-MS) was carried out on WIPO 2 to determine the molecular mass of the compoundsusing (GC-MS)machine. Infrared (IR) spectroscopic analysis was carried out on both WIPO 2 to determine the functional groups present in the compounds using Perkin Elmer Spectrum 100 FTIR Spectrometer.
