The use of bacteria to degrade hydrocarbons from oil-spill sites is one of the most important process of bioremediation of the environment. Oil-spill contaminated samples collected from sewage plants, petrol pumps and harbors were used to get the potential bacterial colonies by pure culture techniques. Different staining techniques were implemented on the isolated bacterial strains for better studying and identification of them. The bacterial colonies so obtained were inoculated in a nutrient broth containing 1ml of oils individually and then mixed oils respectively and the optical density was checked after every 24 hours for 5 days. Finally zone-inhibition test was done to confirm the oil degrading nature of the isolated bacterial colonies.
