To isolate the Acinetobacter species and their antimicrobial resistance pattern. The study was done for a period of one year from July 2013 to June 2014. A total number of 452 patients were included in the study. The culture reports of these patients were analyzed which included ET secretions from patients of end tracheal infections , BAL from pneumonia , Sputum of lower respiratory tract infections patients . The culture which were positive for Acinetobacter spp were identified and their resistance towards antimicrobials was tested. Among 82 strains of Acineobacter spp were obtains comprising of 48 (58.53%) from ET samples ,22 (26.83%) from BAL fluid , 12(14.64%) from Sputum culture .Of these 12 strains were found to be resistance to amikacin . Acinetobacter spp was found to be sensitive to Impenem and Amikacin. Imipenem is found to be very effective in controlling infections caused by Acinetobacter.