Piperine, an alkaloid responsible for the pungency of black pepper & long pepper. Systemic pharmacological studies on Piperine have revealed that this compound elicited diverse pharmacological activities, analgesic, antipyretic, anti-inflammatory, anti-convulsant, CNS-depressant activities & anti-mycobacterial activity. Piperine was isolated from Piper Longum Linn. (Piperaceae). The identity of the compound was confirmed by qualitative analysis and TLC. The purity of the compound was ascertained by UV, FTIR studies and by differential scanning calorimetry (DSC). The anti-mycobacterial activity of the isolated piperine was tested against M. tuberculosis H37Rv strains with standard of Rifampicin by Resazurin Microplate Assay (REMA)
