Yeasts are eukaryotic unicellular organisms and constitute 1% of all described fungal species. Many yeast species are capable of producing different types of bioactive compounds including enzymes viz. amylases, proteases, invertases, lipases, inulinases, cellulose, phytases. The present study was focused on the isolation of lipase producing yeasts from spoiled milk sweet sample collected from Vellore, Tamil Nadu sweet shops. A total of 18 different isolates were recovered from that sample, out of which 14 isolates were selected for primary screening based on distinct colony morphology. Primary screening of the selected yeast isolates was done in tributyrin agar plates where maximum clear zones of inhibition was about 14mm and 18mm respectively, indicating the production of the enzyme lipase. Higher enzyme activity showing 2 isolates were selected and sub-cultured for further. Those 2 yeast isolates were designated as SAGB1 and SAGB2.Secondary screening was done through assay with Phosphate and Tris-HCl buffers. SAGB1 and SAGB2 showed activity 60% and 65% respectively. However, in Tris-HCl buffer they showed activity 58% and 62%. SAGB1 and SAGB2 showed DPPH antioxidant activity of 53.9% and 48.93% respectively. The enzyme was extracted using different solvents as means of partial purification.
